аЯрЁБс>ўџ KMўџџџJџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџьЅС5@ №П‘bjbjЯ2Я2 ) ­X­XKEџџџџџџˆммммммм№єєє8, 8№>ЖXX:’’’’’’"$$$?cѓ*ƒ=$И>R AОЇ=мТ’’ТТЇ=мм’’М=   ТŽм’м’" Т" Z j Њn|ммц’L Рв%‘j4ХєP.ъв=0>ќъШA~pШA$ц№№ммммШAмц(’>а, ќ$ Ђ’’’Ї=Ї=№№єю"№№єInstructions for using the Bran and Luebbe AA3 Check reagents for expiration date and to ensure adequate amount the day before using the AutoAnalyzer. Make reagents as necessary- see method description for directions (found in the “B-L AutoAnalyzer” drawer. Use gloves, goggles and lab coat as needed but especially with phenol. The phenol is said to expire in two weeks, but it is used for much longer than that due to the nastiness of it. Be sure all tubing marked DI or DDW is in bottles with DI water. All other tubing should be in the bottle marked DI+Brij. Be sure waste tubing is in proper waste bottles. Remember to pull the tubing into place and put the cover that attaches over the tubing into place, otherwise the machine will not work. Turn instrument on using power strip and let run for 15min. Check bubbles periodically to make sure they are uniform in size, shape, and frequency. Turn computer on. Click on B+L Aace icon to run program. Click on charting, click on OK and click on YES to download program. This will bring up 2 charts- Channel 1 for NO3, Channel 2 for NH4. Click on SYS window and double click on area in bottom left corner of window to select your method. To set-up the autosampler, click on "Setup", "Analysis" and either (1) copy an existing run or (2) click on your method, hit "OK" and hit "YES" to create a run. Modify the 3 pages, making sure to: Main page- name the file Tray protocol-make sure you have primer, drift, 4-5 calibration standards, QC, blank, pause (so that you can check your calibration curve) all your samples (put QC and blank every 10 samples), QC, blank, drift and end. Channel 1 and 2- Make sure the calibrants concentrations are correct. Click on "Quality Control" and set the range of acceptable values for your QC (+/- 10%). NOTE: Make sure to click “Okay” at the bottom left of the window to save your setup. You will need to reopen it before continuing. Put tubing in reagents (see labels on tubing). Wait 15min. Baseline for both channels should be approx. 5%. If not, click on graph, right click using mouse and left click on "set base". To set the gain. Click on SYS window and double click on "Compact Sampler". Click "Wash" then "Sample". After 30sec-1min, click on "Wash". Repeat this 2 more times and then click on "Cancel". When the first peak appears on the charting window (7min for NH4, 10min for NO3), right click using mouse and left click on "set gain". Repeat for the 2nd peak. Check to make sure the peak is at 85-95% in the 3rd peak. If not repeat step #9-#12. To start the run, click on "Run", "Start", and click on your file. You can view your calibration curve after approx. 15min by clicking on the Cal icon. (The correlation coefficient should have at least three 9’s in a row following the decimal point). Pour all your samples in the cups, making sure to match position on tray with position on tray protocol. When the run is complete a window will pop up letting you know that it is done, click on "OK". (This may take a bit of time). Click on "Retrieve", "Report", and select your file. Make sure data for all of your QC and blanks look okay. Look for drift or carryover. To export your data as a text file, click on "File", "Export to", "ASCII file", click on your file, select the format of your printout, preview it to make sure it looks okay, and hit OK. Use windows explorer to copy the text file to the floppy disk (A:). If running NH4, remove EDTA tubing and place it in DI+Brij. Wait 5min. Remove all tubing from reagents and place in DI+Brij. Run for 15min. Turn off instrument at power strip and turn off computer. Put all reagents back in the refrigerator. Pour NH4 waste in phenol waste and NO3 waste in acid waste containers in hood. NOTE: Please be sure that the cover is taken off of the tubing and the tubing is released from its position so that it is not stretched out. Thanks.  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